产甲烷古菌的甲酰基呋喃脱氢酶所利用的“CO₂”活性物种。
The active species of 'CO2' utilized by formylmethanofuran dehydrogenase from methanogenic Archaea.
作者信息
Vorholt J A, Thauer R K
机构信息
Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany.
出版信息
Eur J Biochem. 1997 Sep 15;248(3):919-24. doi: 10.1111/j.1432-1033.1997.00919.x.
Formylmethanofuran dehydrogenase from methanogenic Archaea catalyzes the reversible conversion of CO2 and methanofuran to formylmethanofuran, which is an intermediate in methanogenesis from CO2, a biological process yielding approximately 0.3 billion tons of CH4 per year. With the enzyme from Methanosarcina barkeri, it is shown that CO2 rather than HCO3- is the active species of 'CO2' utilized by the dehydrogenase. Evidence is also presented that the enzyme catalyzes a methanofuran-dependent exchange between CO2 and the formyl group of formylmethanofuran. The results are consistent with N-carboxymethanofuran being an intermediate in CO2 reduction to formylmethanofuran.
产甲烷古菌中的甲酰基甲烷呋喃脱氢酶催化二氧化碳和甲烷呋喃可逆转化为甲酰基甲烷呋喃，甲酰基甲烷呋喃是二氧化碳生成甲烷过程中的一个中间体，这一生物过程每年产生约3亿吨甲烷。对于巴氏甲烷八叠球菌的这种酶，研究表明脱氢酶所利用的“二氧化碳”的活性形式是二氧化碳而非碳酸氢根。研究还提供证据表明，该酶催化二氧化碳与甲酰基甲烷呋喃的甲酰基之间依赖甲烷呋喃的交换。这些结果与N-羧基甲烷呋喃是二氧化碳还原为甲酰基甲烷呋喃过程中的一个中间体这一观点一致。
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