Stevanato R, Wisniewska A, Momo F
INFM, Department of Physical Chemistry, University of Venice, Italy.
Arch Biochem Biophys. 1997 Oct 15;346(2):203-7. doi: 10.1006/abbi.1997.0253.
Polycationic spermine interacts with the negative phosphate group of dimyristoylphosphatidylglycerol multilamellar liposomes, forming a positively charged shell around the vesicle surface. An association constant of (2.15+/-0.45) x 10(3) M(-1) between spermine and the phospholipid groups in liposomes has been evaluated by a new and rapid enzymatic method. ESR spectra show that the effects of this polycation on liposomes are substantially different from those of cations like Ca2+ and Mg2+ and confirm the ability of spermine to induce liposome aggregation and not fusion.
多阳离子精胺与二肉豆蔻酰磷脂酰甘油多层脂质体的负磷酸基团相互作用，在囊泡表面形成带正电荷的壳层。通过一种新的快速酶法评估了精胺与脂质体中磷脂基团之间的缔合常数为(2.15±0.45)×10³ M⁻¹。电子自旋共振光谱表明，这种多阳离子对脂质体的影响与Ca²⁺和Mg²⁺等阳离子的影响有很大不同，并证实了精胺诱导脂质体聚集而非融合的能力。
