V(D)J重排：体外编码连接的形成
V(D)J recombination: in vitro coding joint formation.
作者信息
Weis-Garcia F, Besmer E, Sawchuk D J, Yu W, Hu Y, Cassard S, Nussenzweig M C, Cortes P
机构信息
Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA.
出版信息
Mol Cell Biol. 1997 Nov;17(11):6379-85. doi: 10.1128/MCB.17.11.6379.
Abstract
Antigen receptor genes are assembled through a mechanism known as V(D)J recombination, which involves two different joining reactions: signal and coding joining. Formation of these joints is essential for antigen receptor assembly as well as maintaining chromosomal integrity. Here we report on a cell-free system for coding joint formation using deletion and inversion recombination substrates. In vitro coding joint formation requires RAG1, RAG2, and heat-labile factors present in the nuclear extract of nonlymphoid cells. Both inversion- and deletion-mediated coding joint reactions produce diverse coding joints, with deletions and P nucleotide addition. We also show that deletion-mediated coding joint formation follows the 12/23 rule and requires the catalytic subunit of DNA-dependent protein kinase.
摘要
抗原受体基因通过一种称为V(D)J重组的机制进行组装，该机制涉及两种不同的连接反应：信号连接和编码连接。这些连接的形成对于抗原受体组装以及维持染色体完整性至关重要。在此，我们报道了一种使用缺失和倒位重组底物进行编码连接形成的无细胞系统。体外编码连接形成需要RAG1、RAG2以及非淋巴细胞核提取物中存在的热不稳定因子。倒位和缺失介导的编码连接反应都会产生多样的编码连接，伴有缺失和P核苷酸添加。我们还表明，缺失介导的编码连接形成遵循12/23规则，并且需要DNA依赖性蛋白激酶的催化亚基。
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本文引用的文献
1
V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins.V(D)J重排：全细胞提取物和DNA弯曲蛋白对RAG1和RAG2在12/23底物上切割活性的调节
J Exp Med. 1997 Jun 2;185(11):2025-32. doi: 10.1084/jem.185.11.2025.
2
RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination.在V(D)J重组过程中，RAG1和RAG2与含有信号末端的DNA形成稳定的切割后突触复合体。
Cell. 1997 Apr 4;89(1):43-53. doi: 10.1016/s0092-8674(00)80181-6.
3
The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo.12/23规则在体内V(D)J重组的切割步骤中起作用。
Genes Cells. 1996 Jun;1(6):543-53. doi: 10.1046/j.1365-2443.1996.d01-259.x.
4
A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage.一种在V(D)J切割中具有活性的稳定RAG1-RAG2-DNA复合物。
Cell. 1997 Jan 10;88(1):65-72. doi: 10.1016/s0092-8674(00)81859-0.
5
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6
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7
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9
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