使用单克隆抗体通过病毒中和试验对几种伪狂犬病病毒株进行鉴定。

Characterization of several pseudorabies viral strains by virus-neutralization test using monoclonal antibodies.

作者信息

Nosetto E O, Echeverria M G, Horimoto T, Pecoraro M R, Galosi C M, Tohya Y, Norimine J, Takahashi E, Etcheverrigaray M E, Mikami T

机构信息

Laboratory of Virology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.

出版信息

Viral Immunol. 1997;10(3):159-64. doi: 10.1089/vim.1997.10.159.

DOI:10.1089/vim.1997.10.159

PMID:9344338

Abstract

In the present study, five mouse monoclonal antibodies (MAbs) to the pseudorabies virus (PRV) Yamagata-81 strain were produced. The MAbs were used in cross-neutralization tests and cross-indirect enzyme-linked immunosorbent assay (ELISA) against three PRV viral strains isolated in Argentina and another four obtained from the United States, Japan, France, and Sweden. Four of five MAbs needed the presence of complement to produce or enhance neutralization activity. No differences were observed by ELISA. The MAbs showed different neutralizing activity against PRV strains, suggesting phenotypic heterogeneity among them.

摘要

在本研究中，制备了针对伪狂犬病病毒（PRV）山形81株的五种小鼠单克隆抗体（MAbs）。这些单克隆抗体用于针对在阿根廷分离的三种PRV病毒株以及从美国、日本、法国和瑞典获得的另外四种病毒株进行交叉中和试验和交叉间接酶联免疫吸附测定（ELISA）。五种单克隆抗体中有四种需要补体的存在才能产生或增强中和活性。通过ELISA未观察到差异。这些单克隆抗体对PRV毒株表现出不同的中和活性，表明它们之间存在表型异质性。