Guignandon A, Usson Y, Laroche N, Lafage-Proust M H, Sabido O, Alexandre C, Vico L
Laboratoire de Biologie du Tissu Osseux, Jean Monnet University, Saint-Etienne, France.
Exp Cell Res. 1997 Oct 10;236(1):66-75. doi: 10.1006/excr.1997.3703.
The relationship between cell morphology and cell metabolism and the role of mechanical load in bone remodeling is well known. Mechanical stimulation induces changes in the shape of osteoblasts, probably mediated by reorganization of focal contacts. We studied the influence of gravity (Gz) variations occurring during parabolic flight on osteoblast focal adhesion of ROS 17/2.8 osteosarcoma cells subjected to 15 or 30 parabolic flights. Significant flight-induced shape changes consisted of decreased cell area associated with focal contact plaque reorganization. Identical durations of continuous mechanical stress induced by centrifugation (2 Gz) or clinorotation (Gz randomization) had no major effect on cell focal adhesion. ROS 17/2.8 G2/M synchronization by treatment with nocodazole inhibited the flight-induced decrease in adhesion parameters. We concluded that ROS 17/2.8 cells are sensitive to Gz switches and that their adaptation is at least dependent on microtubule function.
细胞形态与细胞代谢之间的关系以及机械负荷在骨重塑中的作用是众所周知的。机械刺激会诱导成骨细胞形状发生变化，这可能是由粘着斑的重组介导的。我们研究了抛物线飞行期间发生的重力（Gz）变化对经受15次或30次抛物线飞行的ROS 17/2.8骨肉瘤细胞成骨细胞粘着斑的影响。飞行诱导的显著形状变化包括与粘着斑斑块重组相关的细胞面积减小。通过离心（2 Gz）或clinorotation（Gz随机化）诱导的相同持续时间的连续机械应力对细胞粘着斑没有重大影响。用诺考达唑处理使ROS 17/2.8 G2/M同步化可抑制飞行诱导的粘附参数降低。我们得出结论，ROS 17/2.8细胞对Gz转换敏感，并且它们的适应性至少依赖于微管功能。
