Artola R L, Conde C B, Bagatolli L, Pécora R P, Fidelio G D, Kivatinitz S C
Facultad de Ciencias Químicas;, Universidad Nacional de Córdoba, AP 4, Córdoba, 5000, Argentina.
Biochem Biophys Res Commun. 1997 Oct 20;239(2):570-4. doi: 10.1006/bbrc.1997.7496.
Oxidized lipoproteins have been involved in the pathogenesis of atherosclerosis and atherosclerotic lesions contain oxidized low density lipoprotein. Conversely, the presence of oxidized high density lipoprotein (HDL) in vivo has not been clearly established. Oxidation of HDL in vitro models produces an increase in peroxidized lipids and the appearance of apolipoprotein A-I (apo A-I) oligomers. We investigated the oxidative status of HDL in an in vivo model: the hypercholesterolemic chicken. The HDLs from control and hyperlipemic animals were analyzed for the content of lipid peroxides employing spectroscopic and fluorescence techniques, for the level of apo A-I oligomerization, and for susceptibility to in vitro oxidation. HDL from hypercholesterolemic chickens was more peroxidized (as detected by fluorescence), had higher amount of oligomeric apo A-I, and was oxidized to a greater extent by uv irradiation than that of control animals. We speculate that apo A-I oligomerization could be a key step in the atheroma formation.
氧化型脂蛋白参与了动脉粥样硬化的发病机制，且动脉粥样硬化病变中含有氧化型低密度脂蛋白。相反，体内氧化型高密度脂蛋白（HDL）的存在尚未明确证实。在体外模型中，HDL的氧化会导致过氧化脂质增加以及载脂蛋白A-I（apo A-I）寡聚体的出现。我们在体内模型——高胆固醇血症鸡中研究了HDL的氧化状态。利用光谱和荧光技术分析了对照动物和高脂血症动物的HDL中脂质过氧化物的含量、apo A-I的寡聚化水平以及体外氧化的敏感性。高胆固醇血症鸡的HDL过氧化程度更高（通过荧光检测），具有更高含量的寡聚apo A-I，并且与对照动物相比，经紫外线照射后被氧化的程度更大。我们推测apo A-I寡聚化可能是动脉粥样硬化形成的关键步骤。
