Mangeney M, Beaugé F, Nordmann J, Nordmann R
Arch Int Physiol Biochim. 1979 Aug;87(3):603-12. doi: 10.3109/13813457909070524.
The effects of the various naturally occurring amino acids on ethanol oxidation in hepatocytes from starved rats was systematically studied. In order to minimize the non ADH pathways, the ethanol concentration used was 4 mmol/litre, the amino acids being added at the same concentration. In hepatocytes from fasted rats, alanine, arginine, asparagine, aspartate, citrulline, cysteine, glutamate, glutamine, glycine, histidine, hydroxyproline, ornithine and serine increase significantly ethanol consumption. The stimulatory effect of glutamine being much less pronounced than the asparagine one and proline being devoid of action, the influence of ammonium chloride addition on ethanol consumption in the presence of these amino acids was studied. Ammonium chloride determines an enhancement of ethanol oxidation in these conditions, the results showing no apparent correlation between intracellular glutamate concentration and ethanol oxidation rate, contrarily to previous data. In hepatocytes from fed rats, only alanine, asparagine, cysteine, glycine, hydroxyproline, ornithine and serine increase ethanol oxidation, although to a lesser extent than in cells from starved rats.
系统研究了各种天然存在的氨基酸对饥饿大鼠肝细胞中乙醇氧化的影响。为了尽量减少非乙醇脱氢酶（ADH）途径，所用乙醇浓度为4 mmol/升，氨基酸以相同浓度添加。在禁食大鼠的肝细胞中，丙氨酸、精氨酸、天冬酰胺、天冬氨酸、瓜氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、组氨酸、羟脯氨酸、鸟氨酸和丝氨酸可显著增加乙醇消耗。谷氨酰胺的刺激作用远不如天冬酰胺明显，脯氨酸无作用，因此研究了在这些氨基酸存在下添加氯化铵对乙醇消耗的影响。在这些条件下，氯化铵可增强乙醇氧化，结果表明细胞内谷氨酸浓度与乙醇氧化速率之间无明显相关性，这与先前的数据相反。在喂食大鼠的肝细胞中，只有丙氨酸、天冬酰胺、半胱氨酸、甘氨酸、羟脯氨酸、鸟氨酸和丝氨酸可增加乙醇氧化，尽管程度低于饥饿大鼠细胞。
