Garcia R L, Schilling W P
Rammelkamp Center for Education and Research, Case Western Reserve University School of Medicine, Cleveland, Ohio 44109-1998, USA.
Biochem Biophys Res Commun. 1997 Oct 9;239(1):279-83. doi: 10.1006/bbrc.1997.7458.
Mammalian homologues of the Drosophila trp gene have been invoked as the structural basis for the currents associated with capacitative Ca2+ entry (CCE) in many cell types. Trp homologues are members of a large protein family that may associate as channel subunits providing an explanation for the functional diversity of store-operated channels observed in these cells. However, there is little information as to which of these genes are co-expressed at the cellular level. We have examined the tissue specific expression of five mammalian trp genes and determined which are co-expressed in five different cell lines. The results show tissue- and cell-specific co-expression of multiple trp forms. This implies that the subunit composition of a particular CCE channel may vary depending on the cell type.
果蝇trp基因的哺乳动物同源物已被认为是许多细胞类型中与容量性Ca2+内流（CCE）相关电流的结构基础。Trp同源物是一个大蛋白质家族的成员，它们可能作为通道亚基结合在一起，这为在这些细胞中观察到的储存操纵性通道的功能多样性提供了解释。然而，关于这些基因中哪些在细胞水平上共表达的信息很少。我们研究了五个哺乳动物trp基因的组织特异性表达，并确定了哪些基因在五种不同的细胞系中共表达。结果显示了多种trp形式的组织和细胞特异性共表达。这意味着特定CCE通道的亚基组成可能因细胞类型而异。
