Dellas A, Moch H, Schultheiss E, Feichter G, Almendral A C, Gudat F, Torhorst J
Department of Obstetrics and Gynecology, University of Basel, Switzerland.
Gynecol Oncol. 1997 Oct;67(1):27-33. doi: 10.1006/gyno.1997.4835.
Recently, angiogenic properties have been shown in preinvasive cervical lesions. Our goal was to determine the angiogenesis in cervical intraepithelial neoplasia (CIN) and the relationship between microvessel counts, histopathological parameters, and clinical outcome in invasive cervical carcinoma. One hundred thirty-eight cervical specimens were evaluated; among these 20 were designated normal epithelium, 20 low-grade CIN, 40 high-grade CIN, and 58 invasive carcinoma. Histological sections immunostained for CD31 were quantitatively evaluated for microvessel density. The tumor proliferation rate was determined by the Ki-67 Labeling Index. Comparison of microvessel counts from normal epithelium with those from CIN and invasive carcinoma showed significant increases in precancerous lesions and invasive cancer (P < 0.0001). Microvessel density was found to be associated with the overall survival in women with invasive carcinoma (P < 0.01). There was a significant correlation of microvessel density (P < 0.05) with relapse-free survival in patients with regional lymph node metastasis. A Cox stepwise regression analysis revealed microvessel density, together with depth of invasion, regional lymph node status, and vascular invasion, to be a strong independent prognostic indicator for overall survival in patients with clinical stage IB cervical carcinoma.
最近，在宫颈原位病变中已显示出血管生成特性。我们的目标是确定宫颈上皮内瘤变（CIN）中的血管生成情况，以及微血管计数、组织病理学参数与浸润性宫颈癌临床结局之间的关系。对138例宫颈标本进行了评估；其中20例为正常上皮，20例为低级别CIN，40例为高级别CIN，58例为浸润癌。对免疫染色CD31的组织切片进行微血管密度的定量评估。通过Ki-67标记指数确定肿瘤增殖率。正常上皮与CIN及浸润癌的微血管计数比较显示，癌前病变和浸润癌中的微血管计数显著增加（P < 0.0001）。发现微血管密度与浸润癌女性的总生存期相关（P < 0.01）。微血管密度与区域淋巴结转移患者的无复发生存期显著相关（P < 0.05）。Cox逐步回归分析显示，微血管密度与浸润深度、区域淋巴结状态和血管浸润一起，是临床分期为IB期宫颈癌患者总生存期的强有力独立预后指标。
