Usov A I, Bilan M I, Shashkov A S
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Carbohydr Res. 1997 Aug 25;303(1):93-102. doi: 10.1016/s0008-6215(97)00136-5.
The structure of a sulfated polysaccharide isolated from the calcareous red alga Corallina pilulifera was studied by methylation analysis before and after desulfation or Smith degradation, as well as by 1D and 2D 1H and 13C NMR spectroscopy. The polysaccharide was shown to consist of D-galactose, L-galactose, 2-O-methyl-L-galactose, 3-O-methyl-L-galactose, 6-O-methyl-D-galactose, D-xylose, and sulfate in a molar ratio of 29:20:5:2:1:20:23. Its agaran-like backbone built up of alternating 3-linked beta-D-galactopyranose and 4-linked alpha-L-galactopyranose residues bears single beta-D-xylopyranosyl substituents at position 6 of beta-D-galactose residues, whereas sulfate and O-methyl groups occupy positions 2 and 3 of alpha-L-galactose and position 6 of beta-D-galactose residues.
通过甲基化分析、脱硫或史密斯降解前后的情况以及一维和二维氢谱与碳谱核磁共振光谱，对从钙质红藻珊瑚藻中分离出的一种硫酸化多糖的结构进行了研究。结果表明，该多糖由D-半乳糖、L-半乳糖、2-O-甲基-L-半乳糖、3-O-甲基-L-半乳糖、6-O-甲基-D-半乳糖、D-木糖和硫酸盐组成，摩尔比为29:20:5:2:1:20:23。其类似琼脂糖的主链由交替的3-连接的β-D-吡喃半乳糖和4-连接的α-L-吡喃半乳糖残基构成，在β-D-半乳糖残基的6位带有单个β-D-吡喃木糖基取代基，而硫酸盐和O-甲基基团占据α-L-半乳糖的2位和3位以及β-D-半乳糖残基的6位。
