Fleckenstein A E, Metzger R R, Beyeler M L, Gibb J W, Hanson G R
Department of Pharmacology and Toxicology, University of Utah, Salt Lake City 84112, USA.
Eur J Pharmacol. 1997 Sep 3;334(1):111-4. doi: 10.1016/s0014-2999(97)01175-8.
Incubation of striatal synaptosomes with the oxygen radical generating enzyme, xanthine oxidase, decreased [3H]dopamine uptake: an effect attributable to a decreased Vmax. Concurrent incubation with the superoxide radical scavenger, superoxide dismutase, abolished the xanthine oxidase-induced decrease. These results indicate that, like methamphetamine administration in vivo, reactive oxygen species diminish dopamine transporter function in vitro. The significance of these findings to mechanisms responsible for effects of methamphetamine is discussed.
用产生活性氧的酶黄嘌呤氧化酶孵育纹状体突触体，会降低[3H]多巴胺摄取：这种效应归因于Vmax降低。与超氧化物自由基清除剂超氧化物歧化酶同时孵育，可消除黄嘌呤氧化酶诱导的降低作用。这些结果表明，与体内给予甲基苯丙胺一样，活性氧在体外会削弱多巴胺转运体功能。本文讨论了这些发现对甲基苯丙胺作用机制的意义。
