与人类组蛋白去乙酰化酶基因家族另一个成员相对应的cDNA的分离与鉴定。

Isolation and characterization of cDNAs corresponding to an additional member of the human histone deacetylase gene family.

作者信息

Yang W M, Yao Y L, Sun J M, Davie J R, Seto E

机构信息

Moffitt Cancer Center and Research Institute, the Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa, Florida 33612, USA.

出版信息

J Biol Chem. 1997 Oct 31;272(44):28001-7. doi: 10.1074/jbc.272.44.28001.

DOI:10.1074/jbc.272.44.28001

PMID:9346952

Abstract

Several human cDNAs encoding a histone deacetylase protein, HDAC3, have been isolated. Analysis of the predicted amino acid sequence of HDAC3 revealed an open reading frame of 428 amino acids with a predicted molecular mass of 49 kDa. The HDAC3 protein is 50% identical in DNA sequence and 53% identical in protein sequence compared with the previously cloned human HDAC1. Comparison of the HDAC3 sequence with human HDAC2 also yielded similar results, with 51% identity in DNA sequence and 52% identity in protein sequence. The expressed HDAC3 protein is functionally active because it possesses histone deacetylase activity, represses transcription when tethered to a promoter, and binds transcription factor YY1. Similar to HDAC1 and HDAC2, HDAC3 is ubiquitously expressed in many different cell types.

摘要

已经分离出几种编码组蛋白脱乙酰酶蛋白HDAC3的人类cDNA。对HDAC3预测氨基酸序列的分析显示有一个428个氨基酸的开放阅读框，预测分子量为49 kDa。与先前克隆的人类HDAC1相比，HDAC3蛋白在DNA序列上有50%的同一性，在蛋白质序列上有53%的同一性。将HDAC3序列与人类HDAC2进行比较也得到了类似的结果，在DNA序列上有51%的同一性，在蛋白质序列上有52%的同一性。所表达的HDAC3蛋白具有功能活性，因为它具有组蛋白脱乙酰酶活性，当与启动子相连时可抑制转录，并能结合转录因子YY1。与HDAC1和HDAC2相似，HDAC3在许多不同细胞类型中普遍表达。