Shvarev Y N, Canlon B
Behavioral Phenogenetics Laboratory, Institute for Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk.
Neurosci Lett. 1997 Sep 26;234(1):55-8. doi: 10.1016/s0304-3940(97)00672-1.
The receptor potential as a function of pre-stimulus position of the stereocilia was studied with the whole cell patch-clamp technique in isolated outer hair cells (OHC) during direct mechanical stimulation of the stereocilia (n = 6, cells ranging from 70-87 microm length). The amplitude and frequency of the stimulation was 1 microm and 100 Hz. Controlled pre-stimulus stereocilia displacements in the excitatory direction were followed by sustained membrane depolarization and a diminution of the DC component of the receptor potential. Spectral analysis has shown that recordings with most diminished DC components had a pronounced fundamental while the other harmonics were suppressed, indicating the linearization of the receptor potential. The observed non-lineararities depended on the resting position of the stereocilia and seemed to be determined by the operating range of the transduction process.
采用全细胞膜片钳技术，在直接机械刺激静纤毛的过程中，对分离的外毛细胞（OHC，细胞长度在70 - 87微米之间，n = 6）静纤毛刺激前位置与感受器电位的关系进行了研究。刺激的幅度和频率分别为1微米和100赫兹。在兴奋性方向上对静纤毛进行可控的刺激前位移后，会出现持续的膜去极化以及感受器电位直流成分的减小。频谱分析表明，直流成分减小最多的记录具有明显的基频，而其他谐波被抑制，这表明感受器电位的线性化。观察到的非线性取决于静纤毛的静息位置，似乎由转导过程的工作范围决定。
