从牛虹膜和睫状体中分离出的致葡萄膜炎蛋白。

Uveitogenic proteins isolated from bovine iris and ciliary body.

作者信息

Simpson S C, Kaplan H J, Bora N S

机构信息

Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA.

出版信息

Eye (Lond). 1997;11 ( Pt 2):206-8. doi: 10.1038/eye.1997.52.

DOI:10.1038/eye.1997.52

PMID:9349413

Abstract

Experimental autoimmune anterior uveitis (EAAU) is an animal model for acute anterior uveitis in humans. Previously, we have shown that EAAU can be induced in Lewis rats by a protein(s) associated with ocular melanin. The present study was designed to further purify the pathogenic antigen. Melanin associated antigen (MAA), isolated from bovine iris and ciliary body, was digested with V8 protease and the soluble protein separated on a cation exchange column. The bound protein was eluted with a salt gradient. Lewis rats were immunised with the resulting fractions to test for pathogenicity. Moderate to severe EAAU, with clinical and histopathological features similar to induction with crude soluble bovine MAA, was observed with the 100-200 mM gradient of NH4Cl. Thus, bovine MAA has been partially purified using cation exchange chromatography. Studies are currently under way to purify bovine MAA to homogeneity.

摘要

实验性自身免疫性前葡萄膜炎（EAAU）是人类急性前葡萄膜炎的动物模型。此前，我们已经表明，与眼黑色素相关的一种或多种蛋白质可在Lewis大鼠中诱导EAAU。本研究旨在进一步纯化致病抗原。从牛虹膜和睫状体中分离出的黑色素相关抗原（MAA）用V8蛋白酶消化，可溶性蛋白在阳离子交换柱上分离。结合蛋白用盐梯度洗脱。用所得组分免疫Lewis大鼠以测试其致病性。在100 - 200 mM NH4Cl梯度下，观察到中度至重度EAAU，其临床和组织病理学特征与用粗可溶性牛MAA诱导的相似。因此，已使用阳离子交换色谱法对牛MAA进行了部分纯化。目前正在进行研究以将牛MAA纯化至同质状态。