Kim M C, Kabeer N H, Tandhasetti M T, Kaplan H J, Bora N S
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA.
Curr Eye Res. 1995 Aug;14(8):703-10. doi: 10.3109/02713689508998498.
Experimental autoimmune anterior uveitis (EAAU), a model of uveitis induced by sensitization to melanin associated antigen (MAA) derived from the iris and ciliary body, closely resembles human acute anterior uveitis. The immunopathogenesis of EAAU was studied by immunohistochemical detection of immune cells and the expression of Ia, ICAM-1 and LFA-1 antigens. Male Lewis rats were immunized with bovine MAA, mixed with CFA and pertussis toxin in the hind foot pad. Animals were examined daily by slit-lamp biomicroscopy and serially sacrificed up to 30 days. Immunohistology of the enucleated eyes was performed with monoclonal antibodies W3/25 (CD4), OX-8 (CD8), ED2 (macrophage), OX-33 (B cell), OX-6 (Ia), IA29 (ICAM-1) and WT.1 (LFA-1). During each stage of EAAU, CD4+ T cells predominated over both CD8+ T cells and macrophages in the uvea. Very few B cells were detected during each stage of EAAU. EAAU could not be induced by the adoptive transfer of sera obtained from immunized animals. Low levels of constitutive ICAM-1 and Ia were observed. An increase in ICAM-1 expression was first noted on the epithelial cells of the uveal tract and RPE on day 9 post immunization and preceded LFA-1 and Ia upregulation by approximately 2 days. The immunopathogenesis of EAAU appears to be linked to the presence of the CD4+ T cells.
实验性自身免疫性前葡萄膜炎（EAAU）是一种通过对源自虹膜和睫状体的黑色素相关抗原（MAA）致敏诱导的葡萄膜炎模型，与人类急性前葡萄膜炎极为相似。通过免疫组化检测免疫细胞以及Ia、ICAM - 1和LFA - 1抗原的表达，研究了EAAU的免疫发病机制。雄性Lewis大鼠后足垫注射牛MAA与弗氏完全佐剂（CFA）和百日咳毒素的混合物进行免疫。每天用裂隙灯生物显微镜检查动物，并在30天内分批处死。用单克隆抗体W3/25（CD4）、OX - 8（CD8）、ED2（巨噬细胞）、OX - 33（B细胞）、OX - 6（Ia）、IA29（ICAM - 1）和WT.1（LFA - 1）对摘除的眼球进行免疫组织学检查。在EAAU的每个阶段，葡萄膜中CD4 + T细胞均多于CD8 + T细胞和巨噬细胞。在EAAU的每个阶段均检测到极少的B细胞。从免疫动物获得的血清进行过继转移不能诱导EAAU。观察到组成型ICAM - 1和Ia水平较低。免疫后第9天，首次发现ICAM - 1表达增加，出现在葡萄膜和视网膜色素上皮（RPE）的上皮细胞上，比LFA - 1和Ia上调提前约2天。EAAU的免疫发病机制似乎与CD4 + T细胞的存在有关。
