Bora N S, Woon M D, Tandhasetti M T, Cirrito T P, Kaplan H J
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Invest Ophthalmol Vis Sci. 1997 Sep;38(10):2171-5.
Experimental autoimmune anterior uveitis (EAAU) is an organ-specific autoimmune disease induced by immunization with bovine melanin-associated antigen (MAA) and two adjuvants (complete Freund's adjuvant and purified pertussis toxin). This study was undertaken to explore whether an adjuvant is required in the induction of EAAU.
Insoluble MAA was extracted from the bovine iris and ciliary body. Soluble bovine MAA was derived by treatment of insoluble MAA with the proteolytic enzyme, V8 protease. Lewis rats were immunized with the insoluble or soluble antigen, with or without adjuvant (complete Freund's adjuvant and purified pertussis toxin). Adoptive transfer of CD4+ and CD8+ T cells was performed to investigate the pathogenesis of EAAU.
Experimental autoimmune anterior uveitis can be induced in Lewis rats by immunization with 100 g insoluble bovine MAA alone without the use of adjuvants. The disease can be adoptively transferred to naive syngenic rats by primed CD4+ T cells. In contrast, soluble bovine MAA was not uveitogenic unless adjuvants were employed.
The data suggest that EAAU can be induced in the Lewis rat without addition of an adjuvant. Future studies concerning the pathogenesis of EAAU can now be performed without the possible confounding effect of an adjuvant.
实验性自身免疫性前葡萄膜炎（EAAU）是一种通过用牛黑色素相关抗原（MAA）和两种佐剂（完全弗氏佐剂和纯化百日咳毒素）免疫诱导的器官特异性自身免疫性疾病。本研究旨在探讨诱导EAAU是否需要佐剂。
从不溶性牛虹膜和睫状体中提取MAA。通过用蛋白水解酶V8蛋白酶处理不溶性MAA来获得可溶性牛MAA。用不溶性或可溶性抗原免疫Lewis大鼠，使用或不使用佐剂（完全弗氏佐剂和纯化百日咳毒素）。进行CD4+和CD8+T细胞的过继转移以研究EAAU的发病机制。
单独用100μg不溶性牛MAA免疫Lewis大鼠，无需使用佐剂即可诱导实验性自身免疫性前葡萄膜炎。该疾病可通过致敏的CD4+T细胞过继转移至同基因的未致敏大鼠。相比之下，可溶性牛MAA除非使用佐剂，否则不会诱发葡萄膜炎。
数据表明，无需添加佐剂即可在Lewis大鼠中诱导EAAU。现在可以在没有佐剂可能产生的混杂效应的情况下进行有关EAAU发病机制的未来研究。
