Niederhoffer N, Bobryshev Y V, Lartaud-Idjouadiene I, Giummelly P, Atkinson J
Laboratoire de Pharmacologie Cardio-vasculaire, Faculté de Pharmacie de l'Université Henri-Poincaré, Nancy I, France.
J Vasc Res. 1997 Sep-Oct;34(5):386-98. doi: 10.1159/000159247.
Calcification of the elastic arteries of the young rat by treatment with vitamin D and nicotine (VDN) has been proposed as an animal model of arterial calcification associated with age and age-related vascular pathology in man. The calcium-binding protein, S-100, which is found in human atherosclerotic lesions was associated with medial calcification of the aorta in VDN rats, especially in cases of severe calcification. Calcification (total calcium content: 366 +/- 87, n = 12 in VDN vs. 24 +/- 2 micromol g(-1) aortic dry weight in controls, n = 13) involved elastocalcinosis leading to elastolysis as revealed by a fall in the amount of desmosine and isodesmosine in the aortic wall (266 +/- 17 and 254 +/- 15 in VDN vs. 655 +/- 56 and 588 +/- 30 microg g(-1) aortic dry weight in controls). The decrease in elastin was associated with an increase in the stiffness of the aortic wall (elastic modulus: 15.1 +/- 1.8 in VDN vs. 6.7 +/- 0.5 10(6) dyn cm(-2) in controls), an increase in end-systolic stress (central systolic aortic pressure: 152 +/- 6 in VDN vs. 136 +/- 2 mm Hg in controls) (at a normotensive mean pressure level) and left ventricular hypertrophy (heart weight/body weight 2.51 +/- 0.10 in VDN vs. 2.24 +/- 0.07 g kg(-1) in controls). In conclusion, the mechanisms and consequences of aortic calcification in VDN show several similarities with calcification occurring in human athero- and arteriosclerosis.
用维生素D和尼古丁（VDN）处理幼年大鼠，可使其弹性动脉发生钙化，这已被提议作为与人类年龄及年龄相关血管病变相关的动脉钙化动物模型。在人类动脉粥样硬化病变中发现的钙结合蛋白S-100与VDN大鼠主动脉的中层钙化有关，尤其是在严重钙化的情况下。钙化（总钙含量：VDN组为366±87，n = 12，对照组主动脉干重为24±2 μmol g⁻¹，n = 13）涉及弹性蛋白钙化，导致弹性蛋白溶解，这可通过主动脉壁中锁链素和异锁链素含量的下降得以揭示（VDN组为266±17和254±15，对照组主动脉干重为655±56和588±30 μg g⁻¹）。弹性蛋白的减少与主动脉壁硬度增加（弹性模量：VDN组为15.1±1.8，对照组为6.7±0.5×10⁶ dyn cm⁻²）、收缩末期应力增加（中心收缩期主动脉压：VDN组为152±6，对照组为136±2 mmHg）（在血压正常的平均压力水平下）以及左心室肥厚（心脏重量/体重：VDN组为2.51±0.10，对照组为2.24±0.07 g kg⁻¹）有关。总之，VDN大鼠主动脉钙化的机制和后果与人类动脉粥样硬化和动脉硬化中发生的钙化有若干相似之处。
