Scarpelli M, Montironi R, Thompson D, Bartels P H
Department of Pathology, University of Ancona, Italy.
Anal Quant Cytol Histol. 1997 Oct;19(5):369-75.
To measure a number of nuclear features in a series of glioblastomas and compare the data with those from a set of anaplastic and low grade astrocytomas.
The material consisted of toluidine blue-stained smears from 13 consecutive cases of glioblastoma. Smears from 12 high grade astrocytomas and 13 low grade fibrillary astrocytomas were used for comparison. Fifty nuclei were measured in each case. Cell images were segmented by an interactive procedure. A set of features representing both morphometric and nuclear texture characteristics was computed.
The use of a discriminant function based on two features related to the gray value distribution resulted in the separation of all low grade astrocytomas from glioblastomas. When the corresponding discriminant function was computed for high grade astrocytomas and the values were plotted against optical density, the glioblastomas formed a group at a greater distance from the low grade astrocytomas than from the high grade astrocytomas. A second discriminant function based on two features allowed complete separation of the glioblastoma cases from the high grade astrocytomas.
In our material, chromatin texture analysis allowed effective separation of astrocytic tumors with different histologic grades of malignancy.
测量一系列胶质母细胞瘤的若干细胞核特征，并将数据与一组间变性星形细胞瘤和低级别星形细胞瘤的数据进行比较。
材料包括13例连续胶质母细胞瘤经甲苯胺蓝染色的涂片。使用12例高级别星形细胞瘤和13例低级别纤维性星形细胞瘤的涂片进行比较。每例测量50个细胞核。通过交互式程序对细胞图像进行分割。计算一组代表形态计量学和细胞核纹理特征的特征。
基于与灰度值分布相关的两个特征使用判别函数，可将所有低级别星形细胞瘤与胶质母细胞瘤区分开。当为高级别星形细胞瘤计算相应的判别函数并将值与光密度作图时，胶质母细胞瘤形成的组与低级别星形细胞瘤的距离大于与高级别星形细胞瘤的距离。基于两个特征的第二个判别函数可将胶质母细胞瘤病例与高级别星形细胞瘤完全区分开。
在我们的材料中，染色质纹理分析可有效区分不同组织学恶性级别的星形细胞肿瘤。
