Senior B W
Department of Medical Microbiology, Dundee University Medical School, Ninewells Hospital.
J Med Microbiol. 1997 Jun;46(6):524-7. doi: 10.1099/00222615-46-6-524.
A medium (PAM: Providencia alcalifaciens medium) is described that enables the presence of the enteropathogen P. alcalifaciens in faeces to be detected with ease and simplicity. This organism is probably the only oxidase-negative organism likely to be present in tetrathionate broth cultures of faeces that is unable to ferment the mannitol, xylose or galactose present in the medium. Thus the red colonies of P. alcalifaciens appeared quite distinct from the lemon-yellow acid-forming colonies of all the other bacteria that ferment one or more of these sugars. Extensive tests showed the medium to be both highly specific and sensitive in detecting P. alcalifaciens. Two additional media are described that enable the identity of presumptive P. alcalifaciens isolates to be confirmed unequivocally and with ease.
本文描述了一种培养基（PAM：产碱普罗威登斯菌培养基），它能够轻松、简便地检测粪便中肠道病原菌产碱普罗威登斯菌的存在。该菌可能是粪便硫代硫酸盐肉汤培养物中唯一可能存在的氧化酶阴性菌，且无法发酵培养基中存在的甘露醇、木糖或半乳糖。因此，产碱普罗威登斯菌的红色菌落与所有其他能发酵这些糖中的一种或多种的细菌形成的柠檬黄色酸性菌落截然不同。大量试验表明，该培养基在检测产碱普罗威登斯菌时具有高度特异性和敏感性。还描述了另外两种培养基，它们能够轻松、明确地确认推定的产碱普罗威登斯菌分离株的身份。
