Wang W, Zhuang H, Levitz C L, Fan H, Seldes R M, Tahernia A D, Brighton C T
Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia 19104-6081, USA.
Biochem Mol Biol Int. 1997 Oct;43(2):339-46. doi: 10.1080/15216549700204121.
Mechanical stimulation can prompt healing of bone fractures. However, it is largely unknown how osteogenesis is promoted by mechanical stimulation. In this study, we found that mechanical strain-induced proliferation of osteoblastic cells (MC3T3-E1) accompanied increased levels of platelet-derived growth factor-A (PDGF-A) mRNA, determined by quantitative reverse transcription/polymerase chain reaction. In addition, neomycin and W-7, which blocked mechanical strain-induced proliferation of the osteoblast cells, also blocked mechanical stimulation-induced elevation of PDGF-A mRNA. Finally, an antibody against PDGF can inhibit physical stimulation-induced proliferation of MC3T3-E1 cells, suggesting that the increased MC3T3-E1 cells produced by mechanical stimulation at least partially depends on the increased activity of PDGF.
机械刺激可促使骨折愈合。然而，机械刺激如何促进成骨作用在很大程度上尚不清楚。在本研究中，我们发现，通过定量逆转录/聚合酶链反应测定，机械应变诱导的成骨细胞（MC3T3-E1）增殖伴随着血小板衍生生长因子-A（PDGF-A）mRNA水平的升高。此外，阻断机械应变诱导的成骨细胞增殖的新霉素和W-7，也阻断了机械刺激诱导的PDGF-A mRNA升高。最后，抗PDGF抗体可抑制物理刺激诱导的MC3T3-E1细胞增殖，这表明机械刺激产生的MC3T3-E1细胞增加至少部分依赖于PDGF活性的增加。
