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通过hsp65基因的PCR-限制性片段长度多态性分析快速鉴定分枝杆菌至种水平，并提出一种区分34种分枝杆菌的算法。

Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species.

作者信息

Devallois A, Goh K S, Rastogi N

机构信息

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Point à Pitre, Guadeloupe, French West Indies.

出版信息

J Clin Microbiol. 1997 Nov;35(11):2969-73. doi: 10.1128/jcm.35.11.2969-2973.1997.

DOI:10.1128/jcm.35.11.2969-2973.1997
PMID:9350770
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC230098/
Abstract

PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. C. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was applied to 108 mycobacterial isolates representing 34 species to evaluate its potential as a rapid reference method. A total of 49 distinct patterns were obtained; 25 species were characterized by a single PRA pattern, while 9 species gave more than one specific pattern. An algorithm describing these 34 species (which includes five additional species and new subgroups of Mycobacterium kansasii, M. abscessus, and M. peregrinum) is proposed. A relatively simple and inexpensive method, PRA may be particularly helpful in routine clinical microbiology laboratories.

摘要

应用hsp65基因的聚合酶链反应-限制性片段长度多态性分析（PRA）（A. 泰伦蒂、F. 马尔切西、M. 巴尔兹、F. 巴利、E. C. 博特格和T. 博德默，《临床微生物学杂志》31:175 - 178，1993年）对代表34个菌种的108株分枝杆菌分离株进行检测，以评估其作为一种快速参考方法的潜力。共获得4种不同的模式；25个菌种由单一的PRA模式表征，而9个菌种呈现出不止一种特定模式。提出了一种描述这34个菌种（其中包括另外5个菌种以及堪萨斯分枝杆菌、脓肿分枝杆菌和偶发分枝杆菌的新亚组）的算法。PRA是一种相对简单且成本低廉的方法，可能对常规临床微生物学实验室特别有帮助。

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Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species.通过hsp65基因的PCR-限制性片段长度多态性分析快速鉴定分枝杆菌至种水平，并提出一种区分34种分枝杆菌的算法。
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Routine use of PCR-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media.常规使用聚合酶链反应-限制性片段长度多态性分析来鉴定在液体培养基中生长的分枝杆菌。
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