Ringuet H, Akoua-Koffi C, Honore S, Varnerot A, Vincent V, Berche P, Gaillard J L, Pierre-Audigier C
Service de Microbiologie, Hôpital Necker-Enfants Malades, 75015 Paris, France.
J Clin Microbiol. 1999 Mar;37(3):852-7. doi: 10.1128/JCM.37.3.852-857.1999.
Partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (RGM). A 441-bp fragment (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. Type strains of 10 RGM species were first studied. Each species had a unique nucleotide sequence, distinguishing it clearly from the other species. A panel of strains from the four main RGM species responsible for human infections, Mycobacterium abscessus, Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium peregrinum, was also studied. There were few sequence differences within each of these species (<2% of bases were different from the type strain sequence), and they had no effect on species assignment. hsp65 sequencing unambiguously differentiated M. chelonae and M. abscessus, two species difficult to identify by classical methods and 16S rRNA gene sequencing. The devised procedure is a rapid and reliable tool for the identification of RGM species.
hsp65基因的部分测序用于快速生长分枝杆菌（RGM）的鉴定。通过基于毛细管电泳的自动化荧光法扩增并测序一个441bp的片段（A. 泰伦蒂、F. 马尔切西、M. 巴尔兹、F. 巴利、E. 博特格和T. 博德默，《临床微生物学杂志》31:175 - 178，1993年）。首先研究了10种RGM菌种的模式菌株。每个菌种都有独特的核苷酸序列，能将其与其他菌种清晰区分。还研究了一组来自导致人类感染的四种主要RGM菌种（脓肿分枝杆菌、龟分枝杆菌、偶然分枝杆菌和堪萨斯分枝杆菌）的菌株。这些菌种中的每一个内部序列差异都很少（与模式菌株序列不同的碱基<2%），并且它们对菌种归属没有影响。hsp65测序明确区分了龟分枝杆菌和脓肿分枝杆菌，这两种菌种用经典方法和16S rRNA基因测序很难鉴定。所设计的程序是鉴定RGM菌种的一种快速且可靠的工具。
