Laforgia N, Coppola B, Carbone R, Grassi A, Mautone A, Iolascon A
Dipartimento di Biomedicina dell'Età Evolutiva, Università degli Studi, Bari, Italy.
Acta Paediatr. 1997 Oct;86(10):1097-9. doi: 10.1111/j.1651-2227.1997.tb14815.x.
Clinical diagnosis of sepsis in newborn infants is not easy and there is no laboratory test with 100% specificity and sensitivity, with the exception of blood culture, the results of which are not available for at least 48-72 h. Polymerase chain reaction methodology has been used to diagnose different bacterial, viral and protozoal infections, and the possibility of amplifying the DNA region common to all bacteria could represent an optimal method for the diagnosis of sepsis. The authors have performed PCR in a group of 33 neonates at risk for early-onset sepsis, correlating molecular data with blood culture results. The presence of bacterial DNA in blood samples was evaluated, amplifying the DNA region encoding the 16S rRNA. There were no false negative results (four positive blood cultures and four positive PCR), with competitive costs and time. This method also allows the diagnosis of sepsis due to uncommon species and also, using a second PCR with specific primers, an aetiological diagnosis.
新生儿败血症的临床诊断并不容易，除血培养外，没有一种实验室检查具有100%的特异性和敏感性，而血培养结果至少需要48 - 72小时才能获得。聚合酶链反应方法已被用于诊断不同的细菌、病毒和原生动物感染，扩增所有细菌共有的DNA区域可能是诊断败血症的最佳方法。作者对一组有早发性败血症风险的33例新生儿进行了PCR检测，并将分子数据与血培养结果进行关联。通过扩增编码16S rRNA的DNA区域，评估血样中细菌DNA的存在情况。没有假阴性结果（4例血培养阳性和4例PCR阳性），且成本和时间具有竞争力。该方法还能诊断由不常见菌种引起的败血症，并且通过使用带有特异性引物的第二次PCR进行病因诊断。
