Aarts M G, Hodge R, Kalantidis K, Florack D, Wilson Z A, Mulligan B J, Stiekema W J, Scott R, Pereira A
Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research, AA Wageningen, The Netherlands.
Plant J. 1997 Sep;12(3):615-23. doi: 10.1046/j.1365-313x.1997.00615.x.
The Arabidopsis thaliana MALE STERILITY 2 (MS2) gene product is involved in male gametogenesis. The first abnormalities in pollen development of ms2 mutants are seen at the stage in microsporogenesis when microspores are released from tetrads. Expression of the MS2 gene is observed in tapetum of wild-type flowers at, and shortly after, the release of microspores from tetrads. The MS2 promoter controls GUS expression at a comparable stage in the tapetum of transgenic tobacco containing an MS2 promoter-GUS fusion. The occasional pollen grains produced by mutant ms2 plants have very thin pollen walls. They are also sensitive to acetolysis treatment, which is a test for the presence of an exine layer. The MS2 gene product shows sequence similarity to a jojoba protein that converts wax fatty acids to fatty alcohols. A possible function of the MS2 protein as a fatty acyl reductase in the formation of pollen wall substances is discussed.
拟南芥雄性不育2（MS2）基因产物参与雄配子发生。ms2突变体花粉发育的最初异常出现在小孢子发生阶段，即小孢子从四分体中释放出来的时候。在野生型花朵的绒毡层中，在小孢子从四分体中释放时及之后不久，可观察到MS2基因的表达。MS2启动子在含有MS2启动子 - GUS融合体的转基因烟草绒毡层的类似阶段控制GUS表达。突变的ms2植株偶尔产生的花粉粒具有非常薄的花粉壁。它们对乙酰解处理也很敏感，乙酰解处理是检测外壁层是否存在的一种方法。MS2基因产物与一种将蜡脂肪酸转化为脂肪醇的霍霍巴蛋白具有序列相似性。本文讨论了MS2蛋白作为脂肪酰基还原酶在花粉壁物质形成中的可能功能。
