利用两阶段分批培养法由乳酸乳球菌生产乳酸链球菌素。

Nisin production by Lactococcus lactis using two-phase batch culture.

作者信息

Kim W S

机构信息

Department of Biotechnology, University of New South Wales, Sydney, Australia.

出版信息

Lett Appl Microbiol. 1997 Sep;25(3):169-71. doi: 10.1046/j.1472-765x.1997.00195.x.

DOI:10.1046/j.1472-765x.1997.00195.x

PMID:9351257

Abstract

Nisin production by Lactococcus lactis subsp. lactis C2SmPrt-(TnNip) was investigated using two-phase batch culture. A solvent (phenyl-methyl silicone oil) was introduced in addition to the aqueous phase. The partition coefficient of this solvent in aqueous nisin varied as the cultivation medium changed, with a minimum value being 1.04. The two-phase batch culture supported a 21% increase in growth and a 24% increase in the level of nisin produced compared to the single-phase batch culture.

摘要

使用两相分批培养法研究了乳酸乳球菌乳酸亚种C2SmPrt-(TnNip)产乳链菌肽的情况。除水相外还引入了一种溶剂（苯基甲基硅油）。该溶剂在水相乳链菌肽中的分配系数随培养基的变化而变化，最小值为1.04。与单相分批培养相比，两相分批培养使生长增加了21%，乳链菌肽产量水平提高了24%。