Engel P, Lax A R
US Department of Agriculture, Agricultural Research Service, New Orleans, LA 70124-4305, USA.
Lett Appl Microbiol. 1997 Sep;25(3):225-8. doi: 10.1046/j.1472-765x.1997.00208.x.
Polymerase chain reaction (PCR), Southern hybridization and DNA sequencing experiments were done to determine whether all of Tn4560, a Streptomyces transposon, integrated into the genomes of three nikkomycin non-producing mutants. A deletion of 279 bases occurred at one end of Tn4560 while present in the genome of one of the mutants.