Davies R H, Wray C
Bacteriology Department, Central Veterinary Laboratory, Addlestone, Surrey, UK.
Lett Appl Microbiol. 1997 Oct;25(4):246-8. doi: 10.1046/j.1472-765x.1997.00211.x.
One thousand, four hundred and fifty-one naturally contaminated samples from pig, poultry and cattle farms, poultry hatcheries and animal feed mills were examined in a trial in which transfer of small portions of cellulose sponge coated with salmonella somatic polyvalent antiserum was compared with transfer of standard liquid inocula from pre-enrichment to selective enrichment culture. Salmonella was found in 281 (19.4%) of the samples using the standard method, compared with 385 (26.5%) using the sponge method. It was therefore concluded that antibody-coated cellulose sponges could be a simple means of increasing the recovery of salmonellas from pre-enrichment broths and thereby enhancing the test sensitivity.
在一项试验中，对来自猪、家禽和养牛场、家禽孵化场及动物饲料厂的1451份自然污染样本进行了检测，该试验比较了涂有沙门氏菌菌体多价抗血清的小份纤维素海绵的转移情况与从预增菌培养物到选择性增菌培养物的标准液体接种物的转移情况。使用标准方法在281份（19.4%）样本中检测到沙门氏菌，而使用海绵方法在385份（26.5%）样本中检测到沙门氏菌。因此得出结论，抗体包被的纤维素海绵可能是一种增加从预增菌肉汤中回收沙门氏菌的简单方法，从而提高检测灵敏度。
