Hoszowski A, Truszczyński M
Department of Microbiology, National Veterinary Research Institute, Partyzantów, Poland.
Comp Immunol Microbiol Infect Dis. 1995 Sep;18(4):227-37. doi: 10.1016/0147-9571(95)00015-z.
The purpose of this paper was (1) comparison of four multi-step methods used for Salmonella isolation from meat- and bone powder; (2) elaboration of a new sensitive method of Salmonella isolation from this product; (3) evaluation of a new solid selective medium (BxLH) described by the authors for Salmonella isolation in comparison to brilliant green agar (BGE) according to Edel and Kampelmacher. The study was carried out on 173 meat- and bone powder samples naturally contaminated with Salmonella oranienburg. The samples were examined for the Salmonella presence by means of four compared methods (Methods 1 to 4). The new method of isolation proposed by the authors (Method 3) proved to be the most effective among all compared for Salmonella recovery. It seems that the superiority of Method 3 in comparison to the other applied was a result of, (1) homogenization of the investigated samples in distilled water before preincubation followed by maintenance at room temperature for 2-4 h; (2) the use of a new selective BxLH agar; (3) the use of multiple plating after selective enrichment. The BxLH medium was shown to be more suitable for Salmonella isolation than BGE agar because of more efficient inhibition of other bacterial growth with simultaneously abundant growth of the Salmonella organisms. The additional advantage offered by BxLH agar was the fact that lactose-positive salmonellas grow as typical representatives of this genus. This enables their identification, in contrast to the situation when lactose containing media are used, where the colonies of such salmonellas are similar to the colonies of, for example, Escherichia coli.
（1）比较从肉骨粉中分离沙门氏菌的四种多步方法；（2）精心设计一种从该产品中分离沙门氏菌的新的灵敏方法；（3）评估作者描述的一种新的固体选择培养基（BxLH）用于分离沙门氏菌，并与Edel和Kampelmacher的煌绿琼脂（BGE）作比较。研究在173份自然感染奥兰宁堡沙门氏菌的肉骨粉样本上进行。通过四种比较方法（方法1至4）检测样本中沙门氏菌的存在情况。作者提出的新分离方法（方法3）在所有比较方法中被证明对沙门氏菌的回收率最有效。与其他应用方法相比，方法3的优势似乎源于：（1）在预培养前将被调查样本在蒸馏水中匀浆，然后在室温下保持2 - 4小时；（2）使用新的选择性BxLH琼脂；（3）选择性富集后进行多次平板接种。结果表明，BxLH培养基比BGE琼脂更适合分离沙门氏菌，因为它能更有效地抑制其他细菌生长，同时沙门氏菌大量生长。BxLH琼脂的另一个优势是乳糖阳性沙门氏菌能像该属的典型代表那样生长。这便于它们的鉴定，这与使用含乳糖培养基的情况不同，在含乳糖培养基中，这类沙门氏菌的菌落与例如大肠杆菌的菌落相似。
