从土壤中提取的粗微生物DNA的聚合酶链式反应（PCR）扩增。
PCR amplification of crude microbial DNA extracted from soil.
作者信息
Yeates C, Gillings M R, Davison A D, Altavilla N, Veal D A
机构信息
Key Centre for Biodiversity and Bioresources, School of Biological Sciences, Macquarie University, Sydney, Australia.
出版信息
Lett Appl Microbiol. 1997 Oct;25(4):303-7. doi: 10.1046/j.1472-765x.1997.00232.x.
A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types. DNA was extracted from 100 g of soil using direct lysis with glass beads and sodium dodecyl sulphate (SDS) followed by polyethylene glycol precipitation, potassium acetate precipitation, phenol extraction and isopropanol precipitation. The crude extract could be used in PCR directed at high-copy number (bacterial small subunit rRNA) and single-copy (fungal beta-tubulin) genes.
一种快速、廉价、大规模且纯化步骤极少的DNA提取方法已被开发出来，该方法适用于各种土壤类型。使用玻璃珠和十二烷基硫酸钠（SDS）直接裂解，随后进行聚乙二醇沉淀、醋酸钾沉淀、苯酚提取和异丙醇沉淀，从100克土壤中提取DNA。粗提物可用于针对高拷贝数（细菌小亚基rRNA）和单拷贝（真菌β-微管蛋白）基因的PCR。
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