Briglia M, Eggen R I, de Vos W M, van Elsas J D
Institute for Plant Protection, Wageningen, Netherlands.
Appl Environ Microbiol. 1996 Apr;62(4):1478-80. doi: 10.1128/aem.62.4.1478-1480.1996.
A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.
开发了一种基于16S rRNA基因靶向PCR和寡核苷酸探针的方法,用于检测土壤中的氯酚分枝杆菌PCP-1。引物和探针对三种土壤DNA提取物中的PCP-1具有特异性。该方法能够检测土壤中的PCP-1,检测限为每克3×10(2)个细胞。