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本文引用的文献

1
Bacterial community structure and physiological state within an industrial phenol bioremediation system.工业苯酚生物修复系统中的细菌群落结构与生理状态
Appl Environ Microbiol. 2000 Jun;66(6):2400-7. doi: 10.1128/AEM.66.6.2400-2407.2000.
2
Stable-isotope probing as a tool in microbial ecology.稳定同位素探测作为微生物生态学中的一种工具。
Nature. 2000 Feb 10;403(6770):646-9. doi: 10.1038/35001054.
3
Quantification of bias related to the extraction of DNA directly from soils.与直接从土壤中提取DNA相关的偏差量化。
Appl Environ Microbiol. 1999 Dec;65(12):5409-20. doi: 10.1128/AEM.65.12.5409-5420.1999.
4
Comparison of different methods for the isolation and purification of total community DNA from soil.土壤中总群落DNA分离与纯化的不同方法比较
J Microbiol Methods. 1999 Dec;39(1):1-16. doi: 10.1016/s0167-7012(99)00093-7.
5
Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCR.使用快速RNA提取程序和逆转录-聚合酶链反应（RT-PCR）鉴定淡水沉积物中作为微生物群落活跃成员的新型细菌谱系。
Microbiology (Reading). 1999 Aug;145 ( Pt 8):1977-1987. doi: 10.1099/13500872-145-8-1977.
6
Molecular analysis of bacterial community structure and diversity in unimproved and improved upland grass pastures.未改良和改良高地草原牧场细菌群落结构与多样性的分子分析
Appl Environ Microbiol. 1999 Apr;65(4):1721-30. doi: 10.1128/AEM.65.4.1721-1730.1999.
7
Phylogeny of the main bacterial 16S rRNA sequences in Drentse A grassland soils (The Netherlands).荷兰德伦特省A草原土壤中主要细菌16S rRNA序列的系统发育
Appl Environ Microbiol. 1998 Mar;64(3):871-9. doi: 10.1128/AEM.64.3.871-879.1998.
8
Microbial Evolution, Diversity, and Ecology: A Decade of Ribosomal RNA Analysis of Uncultivated Microorganisms.微生物进化、多样性与生态学：未培养微生物核糖体RNA分析十年
Microb Ecol. 1998 Jan;35(1):1-21. doi: 10.1007/s002489900056.
9
Direct ribosome isolation from soil to extract bacterial rRNA for community analysis.直接从土壤中分离核糖体以提取细菌rRNA用于群落分析。
Appl Environ Microbiol. 1996 Nov;62(11):4162-7. doi: 10.1128/aem.62.11.4162-4167.1996.
10
Direct extraction and purification of rRNA for ecological studies.用于生态研究的rRNA直接提取与纯化
Appl Environ Microbiol. 1993 Mar;59(3):915-8. doi: 10.1128/aem.59.3.915-918.1993.

从自然环境中共提取DNA和RNA以分析基于核糖体DNA和rRNA的微生物群落组成的快速方法。

Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition.

作者信息

Griffiths R I, Whiteley A S, O'Donnell A G, Bailey M J

机构信息

Molecular Microbial Ecology Laboratory, Institute of Virology and Environmental Microbiology, CEH-Oxford, Oxford OX1 3SR, United Kingdom.

出版信息

Appl Environ Microbiol. 2000 Dec;66(12):5488-91. doi: 10.1128/AEM.66.12.5488-5491.2000.

DOI:10.1128/AEM.66.12.5488-5491.2000
PMID:11097934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92488/
Abstract

A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

摘要

本文描述了一种从环境样本中快速提取总核酸的方法。该方法有助于通过从单次提取的样本中进行PCR和逆转录PCR扩增，同时评估微生物16S rRNA多样性。变性梯度凝胶电泳微生物群落分析区分了已建立的草地土壤不同深度处的活性成分（源自rRNA）和总细菌多样性（源自核糖体DNA）。