从自然环境中共提取DNA和RNA以分析基于核糖体DNA和rRNA的微生物群落组成的快速方法。

Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition.

作者信息

Griffiths R I, Whiteley A S, O'Donnell A G, Bailey M J

机构信息

Molecular Microbial Ecology Laboratory, Institute of Virology and Environmental Microbiology, CEH-Oxford, Oxford OX1 3SR, United Kingdom.

出版信息

Appl Environ Microbiol. 2000 Dec;66(12):5488-91. doi: 10.1128/AEM.66.12.5488-5491.2000.

DOI:10.1128/AEM.66.12.5488-5491.2000

PMID:11097934

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92488/

Abstract

A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

摘要

本文描述了一种从环境样本中快速提取总核酸的方法。该方法有助于通过从单次提取的样本中进行PCR和逆转录PCR扩增，同时评估微生物16S rRNA多样性。变性梯度凝胶电泳微生物群落分析区分了已建立的草地土壤不同深度处的活性成分（源自rRNA）和总细菌多样性（源自核糖体DNA）。