Kalyuzhny A E, Wessendorf M W
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455, USA.
Neuroreport. 1997 Oct 20;8(15):3367-72. doi: 10.1097/00001756-199710200-00035.
It has been proposed that mu-opioid receptors excite neurons in hippocampus and nucleus raphe dorsalis (NRD) by decreasing GABAergic tone. In the present study, we examined whether immunocytochemical evidence of interaction between GABAergic neurons and the mu-opioid receptor could be found in the CNS. Portions of rat brain were sectioned and stained for GABA and for the cloned mu-opioid receptor (MOR1) using two-color immunofluorescence. Neurons double-labeled for GABA and MOR1 were present in hippocampus and NRD, as well as in olfactory bulb, dorsal lateral periaqueductal gray matter, nucleus raphe medianis, nucleus raphe obscurus, and the spinal trigeminal nucleus and tract. We conclude that expression of the mu-opioid receptor by GABAergic neurons is common in the rat CNS.
有人提出，μ-阿片受体通过降低GABA能张力来兴奋海马体和中缝背核（NRD）中的神经元。在本研究中，我们检测了在中枢神经系统（CNS）中是否能找到GABA能神经元与μ-阿片受体之间相互作用的免疫细胞化学证据。将大鼠脑的部分组织切片，并用双色免疫荧光法对GABA和克隆的μ-阿片受体（MOR1）进行染色。海马体、NRD以及嗅球、导水管周围灰质背外侧、中缝正中核、中缝隐核、三叉神经脊束核和脊髓束中均存在GABA和MOR1双标记的神经元。我们得出结论，GABA能神经元表达μ-阿片受体在大鼠中枢神经系统中很常见。
