Garcez L M, Silveira F T, el Harith A, Lainson R, Shaw J J
Leishmaniasis Program, Evandro Chagas Institute, Belém, Pará, Brazil.
Acta Trop. 1997 Oct 14;68(1):65-76. doi: 10.1016/s0001-706x(97)00078-8.
The direct agglutination test (DAT) was used to evaluate the serological response of 150 serum samples taken from 15 captive-bred capuchin monkeys Cebus apella. These animals had been experimentally infected with either L. (Leishmania) amazonensis, L. (Viannia) lainsoni or L. (V.) braziliensis. Monkeys infected with L. (L.) amazonensis or L. (V.) lainsoni were challenged with the homologous parasite one month after their spontaneous cure. DAT antigens were prepared from L. (L.) donovani, L. (L.) amazonensis and L. (V.) braziliensis. Antigens were difficult to standardise and it was impossible to produce an L. (V.) lainsoni antigen as parasites remained aggregated even after trypsinization. The DAT detected significant humoral responses in all the infected monkeys. Titres were higher when homologous antigens were used, especially in secondary responses. This suggests that homologous antigen should be used to detect antibodies in human cutaneous leishmaniasis.
直接凝集试验（DAT）用于评估从15只圈养的卷尾猴（僧帽猴属）采集的150份血清样本的血清学反应。这些动物曾被实验性感染亚马逊利什曼原虫、赖氏利什曼原虫或巴西利什曼原虫。感染亚马逊利什曼原虫或赖氏利什曼原虫的猴子在自然治愈一个月后用同源寄生虫进行攻击。DAT抗原由杜氏利什曼原虫、亚马逊利什曼原虫和巴西利什曼原虫制备。抗原难以标准化，且无法制备赖氏利什曼原虫抗原，因为即使经过胰蛋白酶处理，寄生虫仍会聚集。DAT在所有感染的猴子中检测到显著的体液反应。使用同源抗原时滴度更高，尤其是在二次反应中。这表明在人类皮肤利什曼病中应使用同源抗原检测抗体。
