Laurenti Márcia Dalastra, Passero Luiz Felipe Domingues, Tomokane Thaise Yumie, Francesquini Fernanda de Camargo, Rocha Mussya Cisotto, Gomes Claudia Maria de Castro, Corbett Carlos Eduardo Pereira, Silveira Fernando Tobias
Infectious Diseases Laboratory (LIM-50), Pathology Department, Medical School of São Paulo University, Avenida Dr. Arnaldo, 455-1 Andar, Sala 1209, 01246-903 São Paulo, SP, Brazil.
Leishmaniasis Laboratory, Evandro Chagas Institute, Ministry of Health, Rodovia BR 316 s/n, 67030-000 Ananindeua, PA, Brazil ; Tropical Medicine Nucleus, Pará Federal University, Avenida Generalíssimo Deodoro 92, 66055-240 Belém, PA, Brazil.
Biomed Res Int. 2014;2014:134236. doi: 10.1155/2014/134236. Epub 2014 Aug 28.
The purpose of this study was to characterize the immunopathological response in the skin of S. apella infected with Leishmania (L.) amazonensis and L. (V.) braziliensis parasites, the main causative agents of localized cutaneous leishmaniasis in South America. In infected animals, amastigote forms of L. (L.) amazonensis could be detected till 120 days postinfection (PI), while, in L. (V.) braziliensis infection, parasites could be detected until 180 days PI in the skin sections. CD20(+) cells were detected throughout the experimental time in both groups as well as in CD3(+) cells, which appeared to be activated because high densities of inducible nitric oxide synthase (iNOS(+)) cells were detected at 60 and 90 days PI in both studied groups. After 60 and 120 days PI, decrease in iNOS(+) cells was observed in L. (L.) amazonensis and L. (V.) braziliensis, respectively, which was associated with parasite clearance. Increase in lysozyme(+) cells was observed during the experimental infections, which also can be associated with parasite killing.
本研究的目的是描述感染亚马逊利什曼原虫和巴西利什曼原虫（在南美洲引起局部皮肤利什曼病的主要病原体）的沙蚤皮肤中的免疫病理反应。在受感染动物中，感染后120天可检测到亚马逊利什曼原虫的无鞭毛体形式，而在巴西利什曼原虫感染中，在皮肤切片中直到感染后180天才能检测到寄生虫。在整个实验期间，两组均检测到CD20(+)细胞以及CD3(+)细胞，CD3(+)细胞似乎被激活，因为在两个研究组的感染后60天和90天检测到高密度的诱导型一氧化氮合酶（iNOS(+)）细胞。感染后60天和120天，分别在亚马逊利什曼原虫和巴西利什曼原虫中观察到iNOS(+)细胞减少，这与寄生虫清除有关。在实验感染期间观察到溶菌酶(+)细胞增加，这也可能与杀死寄生虫有关。
