Scanziani E, Paltrinieri S, Boldini M, Grieco V, Monaci C, Giusti A M, Mandelli G
Istituto di Anatomia Patologica Veterinaria e Patologia Aviare, Facoltà di Medicina Veterinaria, Milano, Italy.
J Comp Pathol. 1997 Aug;117(2):127-36. doi: 10.1016/s0021-9975(97)80029-1.
Outbreaks of contagious bovine pleuropneumonia (CBPP) were reported in Lombardy, Northern Italy, at the end of 1990. For the purpose of this study, 54 slaughtered Holstein-Friesian cows showing typical lung lesions of CBPP from which the small colony type of Mycoplasma mycoides subspecies mycoides (M. m. mycoides SC) was isolated, were selected. Thoracic lymph nodes from these animals were sampled for bacteriological, histological and immunohistochemical analysis. Acute, subacute and chronic lesions were observed in 13, 12 and 29 cases, respectively. In the 13 animals showing acute lung lesions, an increased number of macrophages was observed, especially in the subcapsular sinuses, but frequently also in the cortical and medullary sinuses of the thoracic lymph nodes; in all 13 acute cases M. m. mycoides SC antigen was detected immunohistochemically in the cytoplasm of the macrophages. In 10 out of the 12 cases with subacute lung lesions, mycoplasma antigen was observed in macrophages located in sinuses, as well as in those scattered in the lymph node parenchyma. Hyperplasia of germinal centres in follicles was observed histologically in most of the 29 cases with chronic lung lesions. In immunohistochemically labelled sections, the characteristic finding observed in 27 of the chronic cases, was the presence of a variable amount of positive material in the germinal centres. These findings demonstrate the involvement of thoracic lymph nodes in CBPP.
1990年末，意大利北部伦巴第地区报告了传染性牛胸膜肺炎（CBPP）疫情。为了本研究的目的，选择了54头屠宰的荷斯坦-弗里生奶牛，这些奶牛表现出CBPP典型的肺部病变，从中分离出了丝状支原体丝状亚种小菌落型（M. m. mycoides SC）。对这些动物的胸段淋巴结进行采样，用于细菌学、组织学和免疫组织化学分析。分别在13例、12例和29例中观察到急性、亚急性和慢性病变。在13例表现为急性肺部病变的动物中，观察到巨噬细胞数量增加，尤其是在被膜下窦，但在胸段淋巴结的皮质和髓窦中也经常出现；在所有13例急性病例中，通过免疫组织化学在巨噬细胞的细胞质中检测到M. m. mycoides SC抗原。在12例亚急性肺部病变的病例中，有10例在位于窦内以及散在于淋巴结实质中的巨噬细胞中观察到支原体抗原。在29例慢性肺部病变的病例中，大多数病例在组织学上观察到滤泡生发中心增生。在免疫组织化学标记的切片中，在27例慢性病例中观察到的特征性发现是生发中心存在数量不等的阳性物质。这些发现表明胸段淋巴结参与了CBPP。
