Grieco V, Boldini M, Luini M, Finazzi M, Mandelli G, Scanziani E
Facoltà di Medicina Veterinaria, Istituto di Anatomia Patologica e Patologia Aviare, Via Celoria 10, 20133 Milano, Italy.
J Comp Pathol. 2001 Feb-Apr;124(2-3):95-101. doi: 10.1053/jcpa.2000.0433.
Between 1990 and 1993, 61 outbreaks of contagious bovine pleuropneumonia (CBPP) were reported in Lombardy, Northern Italy. In this study, gross pathological examination was carried out on 3129 slaughtered cattle, 716 of which (22.9%) showed typical CBPP pulmonary lesions. Single or multiple renal infarcts at different stages of development were observed in 88 (12.2%) of these 716 cattle. The kidneys of 77 cattle whose lungs showed typical CBPP lesions and were bacteriologically and immunohistochemically positive for the small colony type of Mycoplasma mycoides subspecies mycoides (M. m. mycoides SC) were selected and submitted to histological, immunohistochemical and bacteriological examination. Histologically, in chronic CBPP cases, infarcts were characterized by fibrosis, calcification of cortical tubules and tubular atrophy, accompanied by the presence of interstitial inflammatory infiltrates composed of lymphocytes, plasma cells and histiocytes. M. m. mycoides SC antigen was detected immunohistochemically in 65 (84.4%) of the 77 kidneys examined. The antigen was detected in the lumen of blood vessels and in glomerular cells. Immunolabelled interstitial cells and tubular epithelial cells were seen in chronic cases only. M. m. mycoides SC was isolated from the kidneys of 12 animals (15.6%) and more frequently in cases with renal infarcts. This study confirms previous observations that demonstrated a renal involvement in cases of CBPP. Moreover, the immunohistochemical results indicated that M. m. mycoides SC antigen was frequently detectable in different renal structures and cells in spontaneous cases of CBPP.
1990年至1993年间，意大利北部伦巴第地区报告了61起传染性牛胸膜肺炎（CBPP）疫情。在本研究中，对3129头屠宰牛进行了大体病理检查，其中716头（22.9%）呈现典型的CBPP肺部病变。在这716头牛中，有88头（12.2%）观察到处于不同发育阶段的单个或多个肾梗死。选取了77头肺部呈现典型CBPP病变且在细菌学和免疫组织化学检查中对丝状支原体丝状亚种小菌落型（M. m. mycoides SC）呈阳性的牛的肾脏，进行组织学、免疫组织化学和细菌学检查。组织学上，在慢性CBPP病例中，梗死灶的特征为纤维化、皮质肾小管钙化和肾小管萎缩，伴有由淋巴细胞、浆细胞和组织细胞组成的间质炎性浸润。在所检查的77个肾脏中，有65个（84.4%）通过免疫组织化学检测到M. m. mycoides SC抗原。该抗原在血管腔和肾小球细胞中被检测到。仅在慢性病例中可见免疫标记的间质细胞和肾小管上皮细胞。从12只动物（15.6%）的肾脏中分离出M. m. mycoides SC，且在有肾梗死的病例中分离频率更高。本研究证实了先前的观察结果，即CBPP病例存在肾脏受累情况。此外，免疫组织化学结果表明，在CBPP自然病例中，M. m. mycoides SC抗原在不同的肾脏结构和细胞中经常可检测到。
