Marshall V M, Silva A, Foley M, Cranmer S, Wang L, McColl D J, Kemp D J, Coppel R L
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Infect Immun. 1997 Nov;65(11):4460-7. doi: 10.1128/iai.65.11.4460-4467.1997.
Merozoite surface proteins of Plasmodium falciparum play a critical role in the invasion of human erythrocytes by the malaria parasite. Here we describe the identification of a novel protein with a molecular mass of 40 kDa that is found on the merozoite surface of P. falciparum. We call this protein merozoite surface protein 4 (MSP-4). Evidence for the surface location of MSP-4 includes (i) a staining pattern that is consistent with merozoite surface location in indirect immunofluorescent studies of cultured parasites, (ii) localization of MSP-4 in the detergent phase in Triton X-114 partitioning studies, and (iii) nucleotide sequencing studies which predict the presence of an N-terminal signal sequence and a hydrophobic C-terminal sequence in the protein. Immunoprecipitation studies of biosynthetically labelled parasites with [3H] myristic acid indicated that MSP-4 is anchored on the merozoite surface by a glycosylphosphatidylinositol moiety. Of considerable interest is the presence of a single epidermal growth factor-like domain at the C terminus of the MSP-4 protein, making it the second protein with such a structure to be found on the merozoite surface.
恶性疟原虫的裂殖子表面蛋白在疟原虫入侵人类红细胞过程中起关键作用。在此，我们描述了一种分子量为40 kDa的新型蛋白的鉴定，该蛋白存在于恶性疟原虫的裂殖子表面。我们将此蛋白称为裂殖子表面蛋白4（MSP - 4）。MSP - 4位于表面的证据包括：（i）在培养寄生虫的间接免疫荧光研究中，其染色模式与裂殖子表面定位一致；（ii）在Triton X - 114分配研究中，MSP - 4定位于去污剂相；（iii）核苷酸测序研究预测该蛋白存在N端信号序列和疏水的C端序列。用[3H]肉豆蔻酸对生物合成标记的寄生虫进行免疫沉淀研究表明，MSP - 4通过糖基磷脂酰肌醇部分锚定在裂殖子表面。值得关注的是，MSP - 4蛋白的C端存在一个单一的表皮生长因子样结构域，这使其成为在裂殖子表面发现的第二种具有这种结构的蛋白。
