Stutz F, Kantor J, Zhang D, McCarthy T, Neville M, Rosbash M
Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA.
Genes Dev. 1997 Nov 1;11(21):2857-68. doi: 10.1101/gad.11.21.2857.
The FG-repeat domain of the yeast Rip1 protein (Rip1p) was identified initially as a possible target for the nuclear export signal (NES) of the HIV-1 Rev protein in a yeast two-hybrid assay. Rip1p is inessential, associated with nuclear pore complexes, and structurally related to the FG-nucleoporin family of pore proteins. It contributes to HIV-1 Rev-mediated RNA export and is also important for the export of heat shock RNAs at 42 degrees C. We show here that Rip1p is essential for the export of heat shock RNAs, and this function is fulfilled by the unique carboxyl terminus of Rip1p with no substantial contribution from the FG-repeat region. Genetic interactions between Rip1p and the RNA export mediator Gle1p are described, which support a role of the carboxyl terminus of Rip1p in poly(A)+ RNA export. Finally, this domain of Rip1p also contributes to Rev-mediated RNA export. The data suggest that Rip1p promotes the nuclear export of different classes of substrates by contributing to optimal pore function.
酵母Rip1蛋白（Rip1p）的FG重复结构域最初是在酵母双杂交实验中被鉴定为HIV-1 Rev蛋白核输出信号（NES）的一个可能靶点。Rip1p并非必需蛋白，与核孔复合体相关，并且在结构上与孔蛋白的FG核孔蛋白家族相关。它有助于HIV-1 Rev介导的RNA输出，并且对于42摄氏度时热休克RNA的输出也很重要。我们在此表明，Rip1p对于热休克RNA的输出至关重要，并且这种功能由Rip1p独特的羧基末端实现，FG重复区域没有实质性贡献。描述了Rip1p与RNA输出介质Gle1p之间的遗传相互作用，这支持了Rip1p羧基末端在聚腺苷酸（A）+ RNA输出中的作用。最后，Rip1p的这个结构域也有助于Rev介导的RNA输出。数据表明，Rip1p通过促进最佳孔功能来促进不同类底物的核输出。
