Radunović A, Habgood M D, Bradbury M W
Physiology Group, King's College London, UK.
Biometals. 1997 Oct;10(4):343-9. doi: 10.1023/a:1018388803491.
Transferrin and transferrin receptors play an important role in the transport of iron into the brain. To determine whether gallium enters the brain by the same mechanism, uptakes of 67Ga and 59Fe have been compared under controlled conditions. Rates of gallium penetration into brain (K(in)) were four times slower than those for 59Fe. K(in) for 67Ga when infused with citrate were 0.88 +/- 0.24 and 0.94 +/- 0.39 x 10(-3) ml g-1h-1 for cerebral hemisphere and cerebellum, respectively. When infused as the transferrin complex, 67Ga uptake into the brain was not different from that when infused with citrate. The presence of the anti-transferrin receptor antibody OX-26 significantly reduced uptake of 59Fe by 60% and 64% into cerebral hemisphere and cerebellum, respectively. By contrast, pretreatment of rats with OX-26 enhanced the uptake of 67Ga into brain, particularly when infused with citrate; mean increases in uptake of 67Ga were 120% and 144% for cerebral hemisphere and cerebellum, respectively. Purified 67Ga-transferrin was also taken up into both brain regions examined in the presence of OX-26. These results indicate that the transport of non-transferrin bound gallium is an important mechanism for gallium uptake into brain.
转铁蛋白和转铁蛋白受体在铁向脑内的转运中起重要作用。为了确定镓是否通过相同机制进入脑内，在可控条件下比较了67Ga和59Fe的摄取情况。镓进入脑的速率（K（in））比59Fe慢四倍。当与柠檬酸盐一起注入时，67Ga进入大脑半球和小脑的K（in）分别为0.88±0.24和0.94±0.39×10（-3）ml g-1h-1。当作为转铁蛋白复合物注入时，67Ga进入脑内的摄取量与注入柠檬酸盐时无差异。抗转铁蛋白受体抗体OX-26的存在分别使59Fe进入大脑半球和小脑的摄取量显著降低60%和64%。相比之下，用OX-26预处理大鼠可增强67Ga进入脑内的摄取，尤其是在注入柠檬酸盐时；67Ga进入大脑半球和小脑的摄取量平均分别增加120%和144%。在OX-26存在的情况下，纯化的67Ga-转铁蛋白也被摄取到所检测的两个脑区。这些结果表明，非转铁蛋白结合镓的转运是镓进入脑内的重要机制。
