Toyota N, Strebel F R, Stephens L C, Matsuda H, Bull J M
Department of Internal Medicine, University of Texas Medical School, Houston 77225, USA.
Int J Hyperthermia. 1997 Sep-Oct;13(5):497-506. doi: 10.3109/02656739709023548.
This study examines antitumour effect and induction of apoptosis and necrosis after treatment with long-duration, mild whole body hyperthermia (LL-WBH, 40.0 degrees C, for 6 h) in simultaneous combination with cisplatin (CDDP) on primary and metastatic tumour growth in a rat mammary adenocarcinoma. A significantly greater delay in primary mammary tumour growth was observed after treatment with LL-WBH + CDDP, compared to either modality alone (p < 0.05). LL-WBH alone caused a significant delay in spontaneous metastasis to the axillary lymph node (ALN) and LL-WBH + CDDP tended to further increase the delay in ALN metastasis. Survival was longest in rats receiving LL-WBH + CDDP, compared to other groups (p < 0.05). CDDP induced a peak of tumour apoptosis at 24 h after treatment beginning that was significantly greater than LL-WBH alone (p < 0.05). The peak of tumour apoptosis induced by LL-WBH + CDDP from 12 to 24 h was significantly greater than any other group (p < 0.01). These results suggest that the extent of treatment-induced apoptosis seems to correlate positively with antitumour response and the combination or LL-WBH with CDDP may lead to a promising adjuvant therapy for breast cancer.
本研究探讨长时间温和全身热疗（LL-WBH，40.0℃，持续6小时）联合顺铂（CDDP）对大鼠乳腺腺癌原发肿瘤和转移肿瘤生长的抗肿瘤作用以及诱导凋亡和坏死的情况。与单独使用任何一种治疗方式相比，LL-WBH + CDDP治疗后，原发乳腺肿瘤生长的延迟更为显著（p < 0.05）。单独使用LL-WBH可显著延迟自发转移至腋窝淋巴结（ALN），而LL-WBH + CDDP往往会进一步增加ALN转移的延迟。与其他组相比，接受LL-WBH + CDDP治疗的大鼠存活时间最长（p < 0.05）。CDDP在治疗开始后24小时诱导肿瘤凋亡达到峰值，显著高于单独使用LL-WBH（p < 0.05）。LL-WBH + CDDP在12至24小时诱导的肿瘤凋亡峰值显著高于其他任何组（p < 0.01）。这些结果表明，治疗诱导的凋亡程度似乎与抗肿瘤反应呈正相关，LL-WBH与CDDP联合使用可能为乳腺癌带来有前景的辅助治疗方法。
