Tsuboi T, Imada T
Microbiological Diagnosis Laboratory, National Institute of Animal Health, Ibaraki, Japan.
Vet Microbiol. 1997 Sep;57(2-3):135-42. doi: 10.1016/s0378-1135(97)00138-7.
Bovine oocytes enclosed by follicular epithelial (FE) cells were exposed to bovine herpes virus-1 (BHV-1), bluetongue virus (BTV) and akabane virus (AV), matured in culture for 24 h and then in vitro fertilized. In the BHV-1-exposed group, BHV-1 was isolated from medium 3 days after in vitro fertilization at a high titer, and a severe cytopathic effect was observed in the FE cells. Oocytes in the BHV-1-exposed group developed to the 2-cell to 8-cell stage, but failed to develop into blastocysts. In the BTV-exposed group, BTV replication was observed in the FE cells during in vitro embryo development into blastocysts. In the AV-exposed group, no distinct AV replication was detected in FE cells during in vitro embryo development. These results indicate that these viruses failed to infect zona-intact oocytes or embryos during in vitro maturation and culture.
将包裹在卵泡上皮（FE）细胞中的牛卵母细胞暴露于牛疱疹病毒1型（BHV-1）、蓝舌病毒（BTV）和赤羽病病毒（AV），在培养中成熟24小时，然后进行体外受精。在暴露于BHV-1的组中，体外受精3天后从培养基中分离出高滴度的BHV-1，并且在FE细胞中观察到严重的细胞病变效应。暴露于BHV-1组的卵母细胞发育到2细胞至8细胞阶段，但未能发育成囊胚。在暴露于BTV的组中，在体外胚胎发育成囊胚的过程中，在FE细胞中观察到BTV复制。在暴露于AV的组中，在体外胚胎发育过程中，在FE细胞中未检测到明显的AV复制。这些结果表明，这些病毒在体外成熟和培养过程中未能感染完整透明带的卵母细胞或胚胎。
