Rogler G, Andus T, Aschenbrenner E, Vogl D, Falk W, Schölmerich J, Gross V
Department for Internal Medicine I, University of Regensburg, Germany.
Eur J Gastroenterol Hepatol. 1997 Sep;9(9):893-9. doi: 10.1097/00042737-199709000-00013.
Intestinal macrophages play an important role in mucosal inflammation. In normal colonic mucosa we recently demonstrated a unique macrophage phenotype with attenuated immune functions. Here we present an analysis of the alterations of the phenotype of colonic macrophages in inflammatory bowel disease (IBD).
Intestinal macrophages were isolated from biopsies of patients with IBD (n =20). Flow cytometric triple fluorescence analysis was applied to study CD14, CD16, CD33, HLA-DR, CD44, CD11b, CD11c and CD3/CD19 expression.
In IBD there was an increase in expression not only of CD14 compared to control mucosa (36.0% +/- 13.2% vs. 10.5% +/- 3.8%, P< 0.0001) but also of CD16 (28.6% +/- 10.3% vs. 10.1% +/- 3.9%, P< 0.0001), HLA-DR (53.1% +/- 15.9% vs. 27.3% +/- 9.2%, P< 0.0005), CD11b (42.8% +/- 14.2% vs. 17.4% +/- 6.8%, P< 0.0001) and CD11c (35.1% +/- 15.9% vs. 17.8% +/- 10.4%, P< 0.005.). Furthermore, a hitherto undescribed new population of macrophages could be detected by flow cytometry only in patients with ulcerative colitis (CD16++, CD11b++, CD14(low), CD33(low), CD11c-) accounting for 5.8% of all cells isolated.
In contrast to colonic macrophages from normal mucosa, there is a significantly higher expression of CD14, CD16, HLA-DR, CD11b and CD11c in IBD, indicating additional macrophage populations in the inflamed mucosa. This may reflect either a recruitment of new cells from the circulation or a change in phenotype of resident cells.
肠道巨噬细胞在黏膜炎症中起重要作用。在正常结肠黏膜中，我们最近证实了一种具有减弱免疫功能的独特巨噬细胞表型。在此，我们对炎症性肠病（IBD）中结肠巨噬细胞表型的改变进行分析。
从IBD患者（n = 20）的活检组织中分离出肠道巨噬细胞。应用流式细胞术三重荧光分析来研究CD14、CD16、CD33、HLA - DR、CD44、CD11b、CD11c以及CD3/CD19的表达。
在IBD中，与对照黏膜相比，不仅CD14的表达增加（36.0% ± 13.2%对10.5% ± 3.8%，P < 0.0001），而且CD16（28.6% ± 10.3%对10.1% ± 3.9%，P < 0.0001）、HLA - DR（53.1% ± 15.9%对27.3% ± 9.2%，P < 0.0005）、CD11b（42.8% ± 14.2%对17.4% ± 6.8%，P < 0.0001）和CD11c（35.1% ± 15.9%对17.8% ± 10.4%，P < 0.005）的表达也增加。此外，仅在溃疡性结肠炎患者中通过流式细胞术检测到一种迄今未描述的新巨噬细胞群体（CD16++、CD11b++、CD14（低）、CD33（低）、CD11c - ），占分离出的所有细胞的5.8%。
与正常黏膜的结肠巨噬细胞相比，IBD中CD14、CD16、HLA - DR、CD11b和CD11c的表达显著更高，表明炎症黏膜中有额外的巨噬细胞群体。这可能反映了从循环中募集新细胞或驻留细胞表型的改变。
