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人初乳游离分泌片的纯化与特性分析(作者译)

[Purification and characterization of the free secretory piece of human colostrum (author's transl)].

作者信息

Klingmüller D, Hilschmann N

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Dec;360(12):1895-902.

PMID:93565
Abstract

The free secretory piece is isolated from human colostrum by gel filtration and ion-exchange chromatography in high yield (200 mg/l colostrum). DEAE-Cellulose chromatography separates the free secretory piece in two fractions which are electrophoretically distinct, but otherwise have the same characteristics, like molecular weight, antigenic determinants, N-terminal sequence, peptide map and amino acid composition. It was therefore concluded that the protein part of the secretory piece is homogenous.

摘要

通过凝胶过滤和离子交换色谱法从人初乳中以高产率(每升初乳200毫克)分离出游离分泌片。二乙氨基乙基纤维素色谱法将游离分泌片分离成两个电泳性质不同但在其他方面具有相同特征的组分,如分子量、抗原决定簇、N端序列、肽图和氨基酸组成。因此得出结论,分泌片的蛋白质部分是同质的。

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