Xie Z, Fadl A A, Girshick T, Khan M I
Department of Pathobiology, College of Agriculture and Natural Resources, University of Connecticut, Storrs 06269-3089, USA.
Avian Dis. 1997 Jul-Sep;41(3):654-60.
A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven different avian pathogenic viruses and bacteria. As little as 1 pg of avian reovirus RNA was detected using gel electrophoresis and Southern blot hybridization.
建立了一种用于检测禽呼肠孤病毒的逆转录-聚合酶链反应方法。引物来源于禽呼肠孤病毒基因组的S1基因。用这些引物从6个参考毒株和23个禽呼肠孤病毒野毒株中扩增出一条532碱基对的呼肠孤病毒特异性cDNA产物，但从7种不同的禽致病性病毒和细菌中未扩增出该产物。通过凝胶电泳和Southern印迹杂交，可检测到低至1 pg的禽呼肠孤病毒RNA。
