Monteclaro F S, Arai H, Charo I F
Gladstone Institute of Cardiovascular Disease, University of California, San Francisco 94141, USA.
Methods Enzymol. 1997;288:70-84. doi: 10.1016/s0076-6879(97)88008-1.
The construction of chimeric receptors provides a useful starting point for the identification of ligand-binding and G-protein-coupling sites on chemokine receptors. Correlation of the binding and signaling properties of a set of complementary receptor chimeras is a powerful approach for probing structure-function relationships. Further molecular resolution can subsequently be achieved by site-directed mutagenesis and/or alanine scanning.
嵌合受体的构建为确定趋化因子受体上的配体结合位点和G蛋白偶联位点提供了一个有用的起点。一组互补受体嵌合体的结合和信号传导特性的相关性是探究结构-功能关系的有力方法。随后可通过定点诱变和/或丙氨酸扫描实现进一步的分子解析。
