Cupello A, Mainardi P, Robello M, Thellung S
Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy.
Neurochem Res. 1997 Dec;22(12):1517-21. doi: 10.1023/a:1021966814942.
The effect of nitric oxide donors and L-arginine on the uptake of GABA was studied in synaptosomes purified from rat brain. The neurotransmitter uptake was significantly reduced by S-nitrosoacetylpenicillamine and by sodium nitroprusside, although in this case to a lesser extent. A slight inhibitory effect was found preincubating rat brain synaptosomes with 1 mM L-arginine as well. The S-nitrosoacetylpenicillamine effect gradually disappeared with decomposition of the substance by exposure to light. The nitric oxide effect appears to be mainly due to a decrease in the V for synaptosomal GABA uptake and seems to be related to a partial collapse of nerve endings ionic gradients. Functionally, it could result over time in a reduced availability of GABA at the synapses involved.
在从大鼠脑纯化的突触体中研究了一氧化氮供体和L-精氨酸对γ-氨基丁酸（GABA）摄取的影响。S-亚硝基乙酰青霉胺和硝普钠可显著降低神经递质的摄取，不过在后一种情况下降低程度较小。用1 mM L-精氨酸预孵育大鼠脑突触体也发现有轻微的抑制作用。S-亚硝基乙酰青霉胺的作用随着该物质在光照下分解而逐渐消失。一氧化氮的作用似乎主要是由于突触体摄取GABA的V值降低，并且似乎与神经末梢离子梯度的部分崩溃有关。从功能上来说，随着时间的推移，它可能导致相关突触处GABA的可用性降低。
