一种与twist同源的水蛭蛋白：作为母体mRNA遗传的证据。

A leech homolog of twist: evidence for its inheritance as a maternal mRNA.

作者信息

Soto J G, Nelson B H, Weisblat D A

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.

出版信息

Gene. 1997 Oct 15;199(1-2):31-7. doi: 10.1016/s0378-1119(97)00327-2.

DOI:10.1016/s0378-1119(97)00327-2

PMID:9358036

Abstract

In the development of leeches such as Helobdella robusta, mesodermal and ectodermal fates segregate to cells DM and DNOPQ, respectively, at fourth cleavage. As one step in identifying genes that may act in mesoderm determination, we have cloned the H. robusta homolog to the Drosophila gene twist. This homolog, designated Hro-twi, exhibits high (> 90%) amino acid identity with other twist-class genes within its basic-helix loop-helix (b-HLH) DNA binding motif and dimerization domain. Like twist, Hro-twi contains CAX-rich stretches: three stretches 5' to the b-HLH and one located 3' of the b-HLH motif. RT-PCR analysis suggests that Hro-twi is present throughout development, beginning as a maternal transcript in the oocyte.

摘要

在诸如强壮蛭（Helobdella robusta）等水蛭的发育过程中，中胚层和外胚层命运分别在第四次卵裂时分配到细胞DM和DNOPQ。作为鉴定可能参与中胚层决定的基因的一个步骤，我们克隆了强壮蛭与果蝇基因twist的同源物。这个同源物被命名为Hro-twi，在其碱性螺旋-环-螺旋（b-HLH）DNA结合基序和二聚化结构域内与其他twist类基因具有高度（>90%）的氨基酸同一性。与twist一样，Hro-twi含有富含CAX的区域：b-HLH基序5'端有三个区域，b-HLH基序3'端有一个区域。逆转录聚合酶链反应（RT-PCR）分析表明，Hro-twi在整个发育过程中都存在，最初是卵母细胞中的母源转录本。