Giercksky K E, Bjorklid E, Prydz H
Scand J Haematol. 1976 Apr;16(4):300-10. doi: 10.1111/j.1600-0609.1976.tb01155.x.
As a background for the development and testing of phospholipase C in the therapy of post-traumatic and post-surgical intravascular coagulation, highly purified tissue thromboplastin was injected i.v. into rats. The levels of factor V, VII, VIII and blood platelets and the activity of the intrinsic coagulation pathway in general (the cephalin test) were followed. Histological examination of pulmonary, kidney and liver tissue was carried. The dose-response was highly dependent on the injection rate. A marked activation of factor VII and a fall in the activities of factors V and VIII as well as in thrombocyte counts were observed. Very few or no thrombi were seen beyond the pulmonary circulation. The main changes (fibrin-containing thrombi and platelet aggregates) were observed in the lungs during the first 15 min after injection. Atter 15 min virtually no thrombi or platelet aggregates could be detected. The effect of tissue thromboplastin was counteracted by large doses of antithrombin III.
作为在创伤后和手术后血管内凝血治疗中开发和测试磷脂酶C的背景，将高度纯化的组织凝血活酶静脉注射到大鼠体内。跟踪因子V、VII、VIII和血小板的水平以及一般内源性凝血途径的活性（脑磷脂试验）。对肺、肾和肝组织进行了组织学检查。剂量反应高度依赖于注射速率。观察到因子VII明显激活，因子V和VIII的活性以及血小板计数下降。在肺循环之外很少或没有看到血栓。在注射后的前15分钟内在肺部观察到主要变化（含纤维蛋白的血栓和血小板聚集体）。15分钟后几乎检测不到血栓或血小板聚集体。大剂量的抗凝血酶III可抵消组织凝血活酶的作用。
