Ubrich N, Ngondi J, Rivat C, Pfister M, Vigneron C, Maincent P
Laboratoire de Pharmacie Galénique et Biopharmacie, Faculté des Sciences Pharmaceutiques et Biologiques, Nancy, France.
J Biomed Mater Res. 1997 Nov;37(2):155-60. doi: 10.1002/(sici)1097-4636(199711)37:2<155::aid-jbm2>3.0.co;2-q.
Large volume plasma exchanges are used for the removal of anti-A or anti-B antibodies from the plasma of patients undergoing transplantation from donors with major ABO incompatibility. Previous works suggest that solid-phase immunoadsorption can be substituted for plasma exchange in situations where antigens can be purified and immobilized on columns through which plasma is percolated. However, the preparation of purified antigens of the ABO system is large quantities is laborious and requires the use of considerable blood volumes. Studies were therefore undertaken to determine the feasibility of an original immunoadsorbent based on porous microparticles prepared by a water/oil/water emulsification-solvent evaporation method, within which erythrocytes-ghosts carrying blood group antigens were entrapped. The decrease of the antibody hemagglutinating titre after adsorption onto encapsulated ghosts suggests that antibodies can cross the polymeric membrane and bind to the antigens. This original approach of using encapsulated antigens for the batchwise removal of antibodies could be extended to affinity chromatography, and immunoadsorption therapy with a chromatographic column linked to an extracorporeal circulation could be considered.
大容量血浆置换用于从与供体存在主要ABO血型不相容的移植患者血浆中去除抗A或抗B抗体。先前的研究表明，在抗原能够被纯化并固定在柱上，血浆可通过该柱渗滤的情况下，固相免疫吸附可替代血浆置换。然而，大量制备ABO系统的纯化抗原既费力又需要使用大量血液。因此，开展了多项研究，以确定一种基于通过水/油/水乳化-溶剂蒸发法制备的多孔微粒的新型免疫吸附剂的可行性，这些微粒包埋了携带血型抗原的红细胞血影。抗体吸附到包囊血影上后其血凝滴度降低，这表明抗体能够穿过聚合物膜并与抗原结合。这种使用包囊抗原分批去除抗体的创新方法可扩展至亲和色谱，并且可以考虑采用与体外循环相连的色谱柱进行免疫吸附治疗。
