Hoffart Valérie, Maincent Philippe, Lamprecht Alf, Latger-Cannard Véronique, Regnault Véronique, Merle Christian, Jouan-Hureaux Valérie, Lecompte Thomas, Vigneron Claude, Ubrich Nathalie
INSERM U734-EA 3452, Faculty of Pharmacy, Nancy, France.
Pharm Res. 2007 Nov;24(11):2055-62. doi: 10.1007/s11095-007-9340-2. Epub 2007 Jun 14.
Classical immunoadsorbents used for the removal of deleterious molecules in blood such as auto-antibodies are prepared by covalent coupling of antigens onto previously chemically activated supports. Such a chemical treatment may induce a potential toxicity which can be reduced if new immunoadsorbents are prepared by encapsulating erythrocytes-ghosts carrying antigens inside polymeric and porous microparticles.
Erythrocyte-ghosts obtained by hemolysis in hypotonic buffer were encapsulated into ethylcellulose microparticles by w/o/w emulsification. The porosity of microparticles was evaluated by mercury porosimetry. The adsorption ability of encapsulated antigens was evaluated by hemagglutination after contact in tube or elution in column with polyclonal antibody solutions or human blood-plasma.
The encapsulation process did not significantly alter the evaluated antigens since a significant decrease in anti-A (from 256 to 4) as well as anti-Kell (from 64 to 2) antibody titer has been observed in column after eight chromatographic runs (2 h). The higher the ghost concentration (total protein content of 6 mg/ml), the higher the adsorption capacity.
Encapsulation, currently used for drug delivery purposes, may consequently also be applied to the design of new immunoadsorbents as biomaterials.
用于去除血液中有害分子（如自身抗体）的传统免疫吸附剂是通过将抗原共价偶联到预先化学活化的载体上制备的。这种化学处理可能会引发潜在毒性，如果通过将携带抗原的红细胞血影包裹在聚合物多孔微粒中来制备新型免疫吸附剂，这种毒性可能会降低。
通过在低渗缓冲液中溶血获得的红细胞血影通过水包油包水乳化法包裹在乙基纤维素微粒中。通过压汞法评估微粒的孔隙率。通过在试管中与多克隆抗体溶液或人血浆接触或在柱中洗脱后进行血凝试验，评估包裹抗原的吸附能力。
包裹过程并未显著改变所评估的抗原，因为在八次色谱运行（2小时）后，柱中抗A抗体效价（从256降至4）以及抗凯尔抗体效价（从64降至2）均显著降低。血影浓度越高（总蛋白含量为6mg/ml），吸附能力越高。
目前用于药物递送目的的包裹技术因此也可应用于新型免疫吸附剂作为生物材料的设计。
